Carl Zeiss Mikroskopfilter für Fluoreszenzanwendungen
| Modell: |
Filtersatz 62 HE (Colibri) |
| Anregung: |
BP 445/25 (HE)
BP 510/15 (HE)
BP 588/27 (HE) |
| Strahlteiler |
TFT 395 + 495 + 610 (HE) |
| Emission |
TBP 425 + 527 + LP615 (HE) |
Wichtige Hinweise:
Dieser Filtersatz ist nur für die Verwendung mit Lichtquelle Colibri 5 oder 7 geeignet. Er enthält keine
Anregungsfilter. Diese sind in der entsprechenden Colibri Lichtquelle enthalten.
Zusätzliche Komponenten können die Effizienz beeinflussen. Bitte beachten Sie zudem, dass es sich in der
Darstellung um durchschnittliche Werte handelt.
Geeignete Farbstoffe:
| Fluoreszenz-Farbstoffe |
Anregung |
Emission |
| 1,8-ANS
(1-Anilinonaphthalene-8-sulfonic acid) |
375 nm |
480 nm |
| 1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS) |
375 nm |
479 nm |
| 5-FAM pH 9.0 |
492 nm |
518 nm |
| 5-ROX
(5-Carboxy-X-rhodamine, triethylammonium salt) |
578 nm |
604 nm |
| 5-ROX pH 7.0 |
578 nm |
604 nm |
| 6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0 |
358 nm |
450 nm |
| 7-Amino-4-methylcoumarin pH
7.0 |
346 nm |
442 nm |
| 7-Hydroxy-4-methylcoumarin
|
360 nm |
447 nm |
| 7-Hydroxy-4-methylcoumarin
pH 9.0 |
361 nm |
448 nm |
| Alexa 350 |
343 nm |
441 nm |
| Alexa 405 |
401 nm |
421 nm |
| Alexa 430 |
431 nm |
540 nm |
| Alexa 488 |
493 nm |
520 nm |
| Alexa 568 |
576 nm |
603 nm |
| Alexa 594 |
590 nm |
619 nm |
| Alexa Fluor 430
antibody conjugate pH 7.2 |
431 nm |
540 nm |
| Alexa Fluor 488
hydrazide-water |
493 nm |
518 nm |
| Alexa Fluor 568
antibody conjugate pH 7.2 |
579 nm |
603 nm |
| Alexa
Fluor 610 R-phycoerythrin streptavidin pH 7.2 |
567 nm |
627 nm |
| Alexa
Fluor 647 R-phycoerythrin streptavidin pH 7.2 |
569 nm |
666 nm |
| AMCA conjugate |
347 nm |
444 nm |
| Amino Coumarin |
345 nm |
442 nm |
| BCECF pH 5.5 |
485 nm |
521 nm |
| BFP (Blue Fluorescent Protein)
|
380 nm |
439 nm |
| BO-PRO-1-DNA |
462 nm |
482 nm |
| BO-PRO-3-DNA |
574 nm |
604 nm |
| BOBO-1-DNA |
461 nm |
484 nm |
| BOBO-3-DNA |
570 nm |
605 nm |
| BODIPY TR-X phallacidin pH 7.0
|
590 nm |
621 nm |
| BODIPY TR-X, MeOH |
588 nm |
621 nm |
| BODIPY TR-X, SE |
588 nm |
621 nm |
| BOPRO-1 |
462 nm |
482 nm |
| BOPRO-3 |
574 nm |
604 nm |
| Calcein |
493 nm |
514 nm |
| Calcein pH 9.0 |
494 nm |
514 nm |
| Calcium Crimson |
589 nm |
608 nm |
| Calcium Crimson Ca2+ |
590 nm |
608 nm |
| Carboxynaphthofluorescein
pH 10.0 |
600 nm |
674 nm |
| Cascade Blue |
398 nm |
420 nm |
| Cascade Blue BSA pH 7.0 |
401 nm |
419 nm |
| Cascade Yellow |
399 nm |
549 nm |
| Cascade Yellow
antibody conjugate pH 8.0 |
399 nm |
549 nm |
| CFDA |
495 nm |
517 nm |
| CI-NERF pH 2.5 |
504 nm |
541 nm |
| Coumarin |
360 nm |
447 nm |
| Cy 2 |
489 nm |
503 nm |
| Dansyl Cadaverine |
335 nm |
524 nm |
| Dansyl Cadaverine, MeOH |
335 nm |
526 nm |
| DAPI |
358 nm |
463 nm |
| DAPI-DNA |
359 nm |
461 nm |
| Dapoxyl (2-aminoethyl)
sulfonamide |
372 nm |
582 nm |
| DDAO pH 9.0 |
648 nm |
657 nm |
| Di-8 ANEPPS |
469 nm |
630 nm |
| Di-8-ANEPPS-lipid |
469 nm |
631 nm |
| DiO |
487 nm |
501 nm |
| DM-NERF pH 4.0 |
493 nm |
530 nm |
| DTAF |
495 nm |
517 nm |
| DyLight 350 |
332 nm |
435 nm |
| DyLight 405 |
399 nm |
434 nm |
| DyLight 488 |
493 nm |
518 nm |
| DyLight 594 |
592 nm |
616 nm |
| eCFP (Enhanced Cyan
Fluorescent Protein) |
437 nm |
476 nm |
| eGFP (Enhanced
Green Fluorescent Protein) |
488 nm |
509 nm |
| Ethidium homodimer |
528 nm |
617 nm |
| evoglow-Bs1 |
448 nm |
496 nm |
| evoglow-Bs2 |
448 nm |
496 nm |
| evoglow-Pp1 |
448 nm |
495 nm |
| FDA |
495 nm |
517 nm |
| FITC |
495 nm |
517 nm |
| FITC antibody conjugate pH 8.0
|
495 nm |
519 nm |
| Fluo-4 |
494 nm |
516 nm |
| Fluorescein |
495 nm |
517 nm |
| Fluorescein 0.1 M NaOH |
493 nm |
513 nm |
| Fluorescein antibody
conjugate pH 8.0 |
493 nm |
517 nm |
| Fluorescein pH 9.0 |
490 nm |
514 nm |
| Fluoro-Emerald |
495 nm |
524 nm |
| FM 1-43 |
472 nm |
578 nm |
| FM 1-43 lipid |
473 nm |
579 nm |
| FM 4-64 |
508 nm |
751 nm |
| FM 4-64, 2% CHAPS |
506 nm |
751 nm |
| Fura Red, low Ca |
472 nm |
673 nm |
| Fura-2, no Ca |
367 nm |
515 nm |
| GFP (S65T) |
489 nm |
509 nm |
| HcRed |
590 nm |
614 nm |
| Hoechst 33258 |
352 nm |
455 nm |
| Hoechst 33258-DNA |
352 nm |
455 nm |
| Hoechst 33342 |
352 nm |
455 nm |
| Indo-1 Ca2+ |
346 nm |
404 nm |
| Indo-1, Ca free |
346 nm |
479 nm |
| LysoSensor Blue |
374 nm |
424 nm |
| LysoSensor Blue pH 5.0 |
374 nm |
424 nm |
| LysoSensor Green |
447 nm |
504 nm |
| LysoSensor Green pH 5.0 |
447 nm |
502 nm |
| LysoSensor Yellow pH 3.0 |
389 nm |
542 nm |
| LysoSensor Yellow pH 9.0 |
335 nm |
530 nm |
| LysoTracker Blue |
373 nm |
421 nm |
| Marina Blue |
362 nm |
464 nm |
| mCherry |
587 nm |
610 nm |
| mHoneydew |
478 nm |
562 nm |
| MitoTracker Green |
490 nm |
512 nm |
| MitoTracker Green FM, MeOH
|
490 nm |
512 nm |
| mPlum |
587 nm |
649 nm |
| mRFP |
585 nm |
608 nm |
| mStrawberry |
575 nm |
596 nm |
| NBD-X |
466 nm |
534 nm |
| NBD-X, MeOH |
467 nm |
538 nm |
| NeuroTrace 500/525, green fluorescent Nissl stain-RNA |
497 nm |
524 nm |
| Nile Red |
559 nm |
637 nm |
| Nile Red-lipid |
553 nm |
636 nm |
| Nissl |
497 nm |
524 nm |
| Pacific Blue |
404 nm |
455 nm |
| Pacific Blue antibody
conjugate pH 8.0 |
404 nm |
455 nm |
| ReAsH |
597 nm |
608 nm |
| Rhodamine 110 |
497 nm |
520 nm |
| Rhodamine 110 pH 7.0 |
497 nm |
520 nm |
| Rhodamine Green |
497 nm |
524 nm |
| Rhodaminen Green pH 7.0 |
497 nm |
523 nm |
| Rhodol Green antibody
conjugate pH 8.0 |
499 nm |
524 nm |
| Sapphire |
396 nm |
511 nm |
| SYBR Green I |
498 nm |
522 nm |
| SYPRO Ruby |
467 nm |
618 nm |
| SYTO 13-DNA |
488 nm |
506 nm |
| Texas Red-X antibody
conjugate pH 7.2 |
596 nm |
613 nm |
| X-Rhod-1 Ca2+ |
580 nm |
602 nm |
| YO-PRO-1-DNA |
491 nm |
507 nm |
| YO-PRO-3-DNA |
613 nm |
629 nm |
| YOYO-1-DNA |
491 nm |
509 nm |
| YOYO-3-DNA |
612 nm |
631 nm |
- Strahlteiler
- TFT 395 + 495 + 610 (HE)
- Anregung
- BP 370/40 (HE), BP 474/28 (HE), BP 585/35 (HE)
- Emission
- TBP 425 + 527 + LP615 (HE)
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